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Significance of neural antibodies in patients with drug refractory epilepsy
Mojžišová, Hana ; Marusič, Petr (advisor) ; Nytrová, Petra (referee) ; Štourač, Pavel (referee)
Significance of neural antibodies in patients with drug refractory epilepsy Abstract One possible etiology of epilepsy is autoimmune, which was included in the classification of epilepsies in its latest revision published in 2017 by the International League Against Epilepsy. The aim of this study was to evaluate the prevalence of neural autoantibodies in patients with epilepsy and to expand the diagnostic possibilities in the Czech Republic. Together with the Dutch authors, we described the prevalence of neural autoantibodies in Dutch and Czech patients with focal epilepsy of unknown etiology and proposed a scoring system to identify patients prioritized for testing for these antibodies. In a Czech cohort of patients, we assessed the presence of antibodies in serum and CSF of patients with drug refractory epilepsy. The results of both these studies showed a lower prevalence of neural autoantibodies than previously described in patients with epilepsy, due to a more rigorous methodology with verification of positive results by a second method. Since neural autoantibodies are primarily associated with autoimmune encephalitis, we also investigated this group, including an assessment of the presence of epileptic seizures in the clinical profile. Together with the Swedish authors, we investigated the proteomics...
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Effect of diabetogenic autoantigens on the cytokine production of peripheral blood mononuclear cells from type 1 diabetic patients
Labiková, Jana ; Štechová, Kateřina (advisor) ; Holáň, Vladimír (referee)
5 Abstract Type 1 diabetes (T1D) is a serious organ-specific autoimmune disease characterised by irreversible destruction of pancreatic β-cells by immune system. This process results in an absolute insulin deficiency. Both genetical predisposition and environmental factors influence the development of the disease. β-cell destruction is mediated by cellular components of an immune system. Proinflammatory Th1 response is considered as most pathological. Autoimmune destruction of β-cells can be identified by the detection of specific serum autoantibodies a long time before the T1D clinical onset. Currently, there is no efficient cure available to prevent or at least to delay the destructive insulitis. This diploma thesis describes the influence of synthetic diabetogenic autoantigens GAD65 and IA2 on the cytokine response of peripheral blood mononuclear cells (PBMC) obtained from T1D patients with regards to their antibody profile. The study has been carried out on patients with confirmed T1D diagnosis who tested positive for anti-GAD65 and/or anti-IA2 autoantibodies. By using flow cytometry we measured the cell type ratio in PBMC samples. The cells have been stimulated by three different concentrations of antigens and their IFNγ and IL-17 production has been detected by ELISPOT assay. In the case of both...
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Effect of diabetogenic autoantigens on the cytokine production of peripheral blood mononuclear cells from type 1 diabetic patients
Labiková, Jana ; Štechová, Kateřina (advisor) ; Holáň, Vladimír (referee)
5 Abstract Type 1 diabetes (T1D) is a serious organ-specific autoimmune disease characterised by irreversible destruction of pancreatic β-cells by immune system. This process results in an absolute insulin deficiency. Both genetical predisposition and environmental factors influence the development of the disease. β-cell destruction is mediated by cellular components of an immune system. Proinflammatory Th1 response is considered as most pathological. Autoimmune destruction of β-cells can be identified by the detection of specific serum autoantibodies a long time before the T1D clinical onset. Currently, there is no efficient cure available to prevent or at least to delay the destructive insulitis. This diploma thesis describes the influence of synthetic diabetogenic autoantigens GAD65 and IA2 on the cytokine response of peripheral blood mononuclear cells (PBMC) obtained from T1D patients with regards to their antibody profile. The study has been carried out on patients with confirmed T1D diagnosis who tested positive for anti-GAD65 and/or anti-IA2 autoantibodies. By using flow cytometry we measured the cell type ratio in PBMC samples. The cells have been stimulated by three different concentrations of antigens and their IFNγ and IL-17 production has been detected by ELISPOT assay. In the case of both...
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